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HoloCam | LDL | 3DMA | HEW | OFM | P-DH-PALM | DH-PALM | Locate | Track | DH-PSF | QSIP

3D position localization in detector-limited systems

3D point-spread functions can be shaped to increase accuracies over a 3D volume. In the detector-limited regime, the gain in accuracy occurs in all three dimensions throughout the axial range of interest. The PSF is shaped as a double-helix, resulting in a system with fundamentally better 3D localization-accuracies than standard-PSF systems, capable of achieving single-image sub-nanometer accuracies. [See Appl. Phys. Lett. 95 (2009)Proc. SPIE 7184 (2009)]

[See Bright-field DH-PSF Movie]