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Research


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Polarization sensitive 3D superresolution imaging

We demonstrate polarization sensitive detection with 3D super-localization of single-molecules and unveil 3D polarization specific characteristics of single-molecules within the intracellular structure of PtK1 cells expressing photoactivatable green fluorescent protein. The system modulates orthogonal polarization components of single-molecule emissions with a single spatial light modulator and detects them separately with a single detector. Information obtained from the two polarization channels demonstrates polarization based contrast in 3D superresolution imaging. Further, we show that 3D position information from the two channels can be optimally combined to yield up to 30% improvement in localization precision relative to a single polarization channel system. [See Opt. Exp. 17, 19644 (2009)]